beta myosin heavy chain β myhc  (Proteintech)

Journal: Frontiers in Cardiovascular Medicine

Article Title: Metabolic remodeling in cardiac hypertrophy and heart failure with reduced ejection fraction occurs independent of transcription factor EB in mice

doi: 10.3389/fcvm.2023.1323760

Figure Lengend Snippet: Tfeb cKO under pressure overload-induced LVH does not alter cardiac function. ( A ) Kinetics of left ventricular ejection fraction (LVEF), fractional shortening (FS), stroke volume (SV) and cardiac output (CO) as determined by echocardiography at indicated time points after Sham and TAC surgery, respectively, of WT and cKO mice (* = WT_TAC/WT_Sham, “ = cKO_TAC/cKO_Sham, & = cKO_TAC/WT_TAC). ( B ) Heart, lung and liver weights of WT and cKO mice after 21 days of Sham or TAC surgery, normalized to tibia length. ( C–D ) Wheat Germ Agglutinin (WGA) stained histological cross-sections of hearts from WT and cKO mice after 21 days of Sham or TAC surgery. ( D ) Myocyte cross-sectional area (MCSA) measured from on WGA stained sections with Image J. Scale bar, 100 µm. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of Nppa and Nppb ( E ) and Myh6 , Myh7 and Acta1 ( F ) expression from WT and cKO mice after 21 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh . ( G ) Western blot analysis with anti-β-MyHC and anti-GAPDH antibodies. GAPDH was used as loading control. Bar graph showing the ratio of the relative densities of β-MyHC and GAPDH protein contents. ( H ) Representative images of Picrosirius Red stained (PSR, left) heart cross-sections of WT and cKO mice after 21 days of Sham or TAC surgery; scale bar, 1 mm. Fibrotic area (right) was measured with Image J. ( I ) qRT-PCR analysis of Col1a1 , Col1a3 , Fn , and Ctgf from WT and cKO mice after 21 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh . Data are presented as mean ± SD (WT Sham: n = 10, WT TAC: n = 13, cKO Sham: n = 10, cKO TAC: n = 15; *,”,& q < 0.05, ** q < 0.01, *** q < 0.001, **** q < 0.0001).

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) in TBS-T (20 mM Tris, 150 mM NaCl, 0.1% Tween 20; pH 7.6) for 1 h. Following primary antibodies were used: affinity-purified rabbit anti-TFEB antibody A303-673A (RRID: AB_11204751, 1:1000, Bethyl Laboratories, Hamburg, Germany), monoclonal anti-β/slow myosin heavy chain (β-MyHC) (RRID: AB_297734, clone: NOQ7.5.4D, mouse, 1:1,000, Merck KGaA, Munich, Germany).

Techniques: Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Cardiovascular Medicine

Article Title: Metabolic remodeling in cardiac hypertrophy and heart failure with reduced ejection fraction occurs independent of transcription factor EB in mice

doi: 10.3389/fcvm.2023.1323760

Figure Lengend Snippet: Deletion of TFEB reduces cardiac remodeling in pressure overload-induced HFrEF. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of Nppa and Nppb ( A ) and Myh6 , Myh7 , and Acta1 ( B ) expression from WT and cKO mice after 56 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh . ( C ) Western blot analysis with anti-β-MyHC and anti-GAPDH antibodies. GAPDH was used as loading control. Bar graph showing the ratio of the relative densities of β-MyHC and GAPDH protein contents. ( D ) Representative images of Picrosirius Red stained (PSR, left) heart cross-sections of WT and cKO mice after 56 days of Sham or TAC surgery; scale bar, 1 mm. Fibrotic area (right) was measured with Image J. ( E ) qRT-PCR analysis of indicated genes from WT and cKO mice after 56 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh . Data are presented as mean ± SD (56 days, WT Sham: n = 10, WT TAC: n = 6, cKO Sham: n = 8, cKO TAC: n = 6; *,”,& q < 0.05 ** q < 0.01, *** q < 0.001, **** q < 0.0001).

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) in TBS-T (20 mM Tris, 150 mM NaCl, 0.1% Tween 20; pH 7.6) for 1 h. Following primary antibodies were used: affinity-purified rabbit anti-TFEB antibody A303-673A (RRID: AB_11204751, 1:1000, Bethyl Laboratories, Hamburg, Germany), monoclonal anti-β/slow myosin heavy chain (β-MyHC) (RRID: AB_297734, clone: NOQ7.5.4D, mouse, 1:1,000, Merck KGaA, Munich, Germany).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot, Staining